Flexible multiplex PCR to detect SARS-CoV-2, coronavirus OC43 and influenza A virus in nasopharyngeal swab samples
dc.contributor.author | Pelegrí Martínez, Eduardo | |
dc.contributor.author | Guruceaga Sierra, Xabier | |
dc.contributor.author | Martín Souto, Leire | |
dc.contributor.author | Abad Díaz de Cerio, Ana | |
dc.contributor.author | Rementeria Ruiz, Aitor Domingo | |
dc.contributor.author | Domínguez Monedero, Alazne | |
dc.contributor.author | Gallego, Mikel | |
dc.contributor.author | Martínez, Oscar | |
dc.contributor.author | Arana Arri, Eunate | |
dc.contributor.author | Aranzamendi, Maitane | |
dc.contributor.author | Ramírez García, Andoni | |
dc.date.accessioned | 2023-03-15T16:07:22Z | |
dc.date.available | 2023-03-15T16:07:22Z | |
dc.date.issued | 2022-12 | |
dc.identifier.citation | Journal of Applied Microbiology 133(6) : 3534-3545 (2022) | es_ES |
dc.identifier.issn | 1364-5072 | |
dc.identifier.issn | 1365-2672 | |
dc.identifier.uri | http://hdl.handle.net/10810/60360 | |
dc.description.abstract | Introduction Quantitative reverse transcription PCR (RT-qPCR) is the leading tool to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Given that it will almost certainly continue to coexist with other respiratory viruses in the coming years, our study aimed to design a multiplex PCR system not affected by supplier outages and with reduced cost compared to the existing commercially available kits. Methods and results In this study, combinations of four primers/probe sets were used to construct a flexible RT-qPCR assay which is capable of discriminating between SARS-CoV-2 and the seasonal human coronavirus HCoV-OC43, or even influenza A virus. Additionally, the human RPP30 gene was used as an internal control. To demonstrate the robustness of the assay, it was applied to a collection of 150 clinical samples. The results showed 100% sensitivity and specificity compared to the automatized system used at the hospital and were better when indeterminate samples were analysed. Conclusions This study provides an efficient method for the simultaneous detection of SARS-CoV-2, HCoV-OC43 and influenza A virus, and its efficacy has been tested on clinical samples showing outstanding results. Significance and impact of the study The multiplex RT-qPCR design offers an accessible and economical alternative to commercial detection kits for hospitals and laboratories with limited economic resources or facing situations of supply shortage. | es_ES |
dc.description.sponsorship | This research was funded by Basque Government, grants numbers 2020333042 and IT1362‐19. LM‐S have received a predoctoral Grant from Basque Government. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Wiley | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.subject | coronavirus | es_ES |
dc.subject | influenza A virus | es_ES |
dc.subject | multiplex RT-qPCR | es_ES |
dc.subject | OC43 | es_ES |
dc.subject | SARS-CoV-2 | es_ES |
dc.title | Flexible multiplex PCR to detect SARS-CoV-2, coronavirus OC43 and influenza A virus in nasopharyngeal swab samples | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.holder | © 2022 The Authors. Journal of Applied Microbiology published by John Wiley & Sons Ltd on behalf of Society for Applied Microbiology. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. | es_ES |
dc.rights.holder | Atribución 3.0 España | * |
dc.relation.publisherversion | https://academic.oup.com/jambio/article/133/6/3534/6989080?login=true | es_ES |
dc.identifier.doi | 10.1111/jam.15788 | |
dc.departamentoes | Inmunología, microbiología y parasitología | es_ES |
dc.departamentoeu | Immunologia, mikrobiologia eta parasitologia | es_ES |
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Except where otherwise noted, this item's license is described as © 2022 The Authors. Journal of Applied Microbiology published by John Wiley & Sons Ltd on behalf of Society for Applied Microbiology.
This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any
medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.