dc.contributor.author | Gutiérrez Martínez, Enric | |
dc.contributor.author | Benet Garrabé, Susana | |
dc.contributor.author | Mateos, Nicolás | |
dc.contributor.author | Erkizia, Itziar | |
dc.contributor.author | Nieto Garai, Jon Ander | |
dc.contributor.author | Lorizate Nogales, Maier | |
dc.contributor.author | Borgman, Kyra J.E. | |
dc.contributor.author | Manzo, Carlo | |
dc.contributor.author | Campelo, Félix | |
dc.contributor.author | Izquierdo Useros, Nuria | |
dc.contributor.author | Martínez Picado, Javier | |
dc.contributor.author | García Parajo, María F. | |
dc.date.accessioned | 2023-04-21T16:38:49Z | |
dc.date.available | 2023-04-21T16:38:49Z | |
dc.date.issued | 2023-03 | |
dc.identifier.citation | eLife 12 : (2023) // Article ID e78836 | es_ES |
dc.identifier.issn | 2050-084X | |
dc.identifier.uri | http://hdl.handle.net/10810/60886 | |
dc.description.abstract | The immunoglobulin-like lectin receptor CD169 (Siglec-1) mediates the capture of
HIV- 1 by activated dendritic cells (DCs) through binding to sialylated ligands. These interactions
result in a more efficient virus capture as compared to resting DCs, although the underlying mech-
anisms are poorly understood. Using a combination of super-resolution microscopy, single-particle
tracking and biochemical perturbations we studied the nanoscale organization of Siglec-1 on acti-
vated DCs and its impact on viral capture and its trafficking to a single viral-containing compartment.
We found that activation of DCs leads to Siglec-1 basal nanoclustering at specific plasma membrane
regions where receptor diffusion is constrained by Rho-ROCK activation and formin-dependent actin
polymerization. Using liposomes with varying ganglioside concentrations, we further demonstrate
that Siglec-1 nanoclustering enhances the receptor avidity to limiting concentrations of gangliosides
carrying sialic ligands. Binding to either HIV-1 particles or ganglioside-bearing liposomes lead to
enhanced Siglec-1 nanoclustering and global actin rearrangements characterized by a drop in RhoA
activity, facilitating the final accumulation of viral particles in a single sac-like compartment. Overall,
our work provides new insights on the role of the actin machinery of activated DCs in regulating the
formation of basal Siglec-1 nanoclustering, being decisive for the capture and actin-dependent traf-
ficking of HIV-1 into the virus-containing compartment | es_ES |
dc.description.sponsorship | he research
leading to these results has received funding from the European Commission H2020 Program (grant
agreement ERC Adv788546 (NANO-MEMEC) (to MFG-P) and Marie Sklodowska-Curie grant 754558-
PREBIST (to NM)), Government of Spain Severo Ochoa CEX2019-000910-S, State Research Agency
(AEI) (PID2020- 113068RB- I00/10.13039/501100011033 (to MFG- P), PID2019- 109870RB- I00 (to JM- P),
(PID2020- 117405GB-100 to ML), RYC-2017-22227 (to FC), RYC-2015-17896 (to CM), and PID2019-
106232RB-I00/10.13039/501100011033 (to FC)), Fundació CELLEX (Barcelona), Fundació Mir- Puig
and the Generalitat de Catalunya through the CERCA program and AGAUR (Grant No. 2017SGR1000
to MFG-P). NI-U is supported by grant PID2020-117145RB-I00 from the Spanish Ministry of Science
and Innovation. JM- P an NI- U are funded by the CIBER de Enfermedades Infecciosas. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | eLife Sciences | es_ES |
dc.relation | info:eu-repo/grantAgreement/EC/ERC/788546 | es_ES |
dc.relation | info:eu-repo/grantAgreement/EC/H2020/754558 | es_ES |
dc.relation | info:eu-repo/grantAgreement/MICINN/CEX2019-000910-S | es_ES |
dc.relation | info:eu-repo/grantAgreement/MICINN/PID2020-113068RB-I00 | es_ES |
dc.relation | info:eu-repo/grantAgreement/MICINN/PID2019-109870RB-I00 | es_ES |
dc.relation | info:eu-repo/grantAgreement/MICINN/PID2020-117405GB-100 | es_ES |
dc.relation | info:eu-repo/grantAgreement/MINECO/RYC-2017-22227 | es_ES |
dc.relation | info:eu-repo/grantAgreement/MINECO/RYC-2015-17896 | es_ES |
dc.relation | info:eu-repo/grantAgreement/MICINN/PID2019-106232RB-I00 | es_ES |
dc.relation | info:eu-repo/grantAgreement/MICINN/PID2020-117145RB-I00 | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.title | Actin- regulated Siglec- 1 nanoclustering influences HIV-1 capture and virus- containing compartment formation in dendritic cells | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.holder | Copyright Gutiérrez-Martínez
et al. This article is distributed
under the terms of the Creative
Commons Attribution License,
which permits unrestricted use
and redistribution provided that
the original author and source
are credited. | es_ES |
dc.rights.holder | Atribución 3.0 España | * |
dc.relation.publisherversion | https://elifesciences.org/articles/78836 | es_ES |
dc.identifier.doi | 10.7554/eLife.78836 | |
dc.contributor.funder | European Commission | |
dc.departamentoes | Bioquímica y biología molecular | es_ES |
dc.departamentoeu | Biokimika eta biologia molekularra | es_ES |