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dc.contributor.authorGutiérrez Martínez, Enric
dc.contributor.authorBenet Garrabé, Susana
dc.contributor.authorMateos, Nicolás
dc.contributor.authorErkizia, Itziar
dc.contributor.authorNieto Garai, Jon Ander
dc.contributor.authorLorizate Nogales, Maier
dc.contributor.authorBorgman, Kyra J.E.
dc.contributor.authorManzo, Carlo
dc.contributor.authorCampelo, Félix
dc.contributor.authorIzquierdo Useros, Nuria
dc.contributor.authorMartínez Picado, Javier
dc.contributor.authorGarcía Parajo, María F.
dc.date.accessioned2023-04-21T16:38:49Z
dc.date.available2023-04-21T16:38:49Z
dc.date.issued2023-03
dc.identifier.citationeLife 12 : (2023) // Article ID e78836es_ES
dc.identifier.issn2050-084X
dc.identifier.urihttp://hdl.handle.net/10810/60886
dc.description.abstractThe immunoglobulin-like lectin receptor CD169 (Siglec-1) mediates the capture of HIV- 1 by activated dendritic cells (DCs) through binding to sialylated ligands. These interactions result in a more efficient virus capture as compared to resting DCs, although the underlying mech- anisms are poorly understood. Using a combination of super-resolution microscopy, single-particle tracking and biochemical perturbations we studied the nanoscale organization of Siglec-1 on acti- vated DCs and its impact on viral capture and its trafficking to a single viral-containing compartment. We found that activation of DCs leads to Siglec-1 basal nanoclustering at specific plasma membrane regions where receptor diffusion is constrained by Rho-ROCK activation and formin-dependent actin polymerization. Using liposomes with varying ganglioside concentrations, we further demonstrate that Siglec-1 nanoclustering enhances the receptor avidity to limiting concentrations of gangliosides carrying sialic ligands. Binding to either HIV-1 particles or ganglioside-bearing liposomes lead to enhanced Siglec-1 nanoclustering and global actin rearrangements characterized by a drop in RhoA activity, facilitating the final accumulation of viral particles in a single sac-like compartment. Overall, our work provides new insights on the role of the actin machinery of activated DCs in regulating the formation of basal Siglec-1 nanoclustering, being decisive for the capture and actin-dependent traf- ficking of HIV-1 into the virus-containing compartmentes_ES
dc.description.sponsorshiphe research leading to these results has received funding from the European Commission H2020 Program (grant agreement ERC Adv788546 (NANO-MEMEC) (to MFG-P) and Marie Sklodowska-Curie grant 754558- PREBIST (to NM)), Government of Spain Severo Ochoa CEX2019-000910-S, State Research Agency (AEI) (PID2020- 113068RB- I00/10.13039/501100011033 (to MFG- P), PID2019- 109870RB- I00 (to JM- P), (PID2020- 117405GB-100 to ML), RYC-2017-22227 (to FC), RYC-2015-17896 (to CM), and PID2019- 106232RB-I00/10.13039/501100011033 (to FC)), Fundació CELLEX (Barcelona), Fundació Mir- Puig and the Generalitat de Catalunya through the CERCA program and AGAUR (Grant No. 2017SGR1000 to MFG-P). NI-U is supported by grant PID2020-117145RB-I00 from the Spanish Ministry of Science and Innovation. JM- P an NI- U are funded by the CIBER de Enfermedades Infecciosas.es_ES
dc.language.isoenges_ES
dc.publishereLife Scienceses_ES
dc.relationinfo:eu-repo/grantAgreement/EC/ERC/788546es_ES
dc.relationinfo:eu-repo/grantAgreement/EC/H2020/754558es_ES
dc.relationinfo:eu-repo/grantAgreement/MICINN/CEX2019-000910-Ses_ES
dc.relationinfo:eu-repo/grantAgreement/MICINN/PID2020-113068RB-I00es_ES
dc.relationinfo:eu-repo/grantAgreement/MICINN/PID2019-109870RB-I00es_ES
dc.relationinfo:eu-repo/grantAgreement/MICINN/PID2020-117405GB-100es_ES
dc.relationinfo:eu-repo/grantAgreement/MINECO/RYC-2017-22227es_ES
dc.relationinfo:eu-repo/grantAgreement/MINECO/RYC-2015-17896es_ES
dc.relationinfo:eu-repo/grantAgreement/MICINN/PID2019-106232RB-I00es_ES
dc.relationinfo:eu-repo/grantAgreement/MICINN/PID2020-117145RB-I00es_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/es/*
dc.titleActin- regulated Siglec- 1 nanoclustering influences HIV-1 capture and virus- containing compartment formation in dendritic cellses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.holderCopyright Gutiérrez-Martínez et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.es_ES
dc.rights.holderAtribución 3.0 España*
dc.relation.publisherversionhttps://elifesciences.org/articles/78836es_ES
dc.identifier.doi10.7554/eLife.78836
dc.contributor.funderEuropean Commission
dc.departamentoesBioquímica y biología moleculares_ES
dc.departamentoeuBiokimika eta biologia molekularraes_ES


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Copyright Gutiérrez-Martínez
et al. This article is distributed
under the terms of the Creative
Commons Attribution License,
which permits unrestricted use
and redistribution provided that
the original author and source
are credited.
Except where otherwise noted, this item's license is described as Copyright Gutiérrez-Martínez et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.