Effects of a N-Maleimide-derivatized Phosphatidylethanolamine on the Architecture and Properties of Lipid Bilayers
dc.contributor.author | Ballesteros Rivero, Uxue | |
dc.contributor.author | González Ramírez, Emilio José | |
dc.contributor.author | De la Arada Echevarría, Igor ![]() | |
dc.contributor.author | Sot, Jesús | |
dc.contributor.author | Etxaniz Iriondo, Asier | |
dc.contributor.author | Goñi Urcelay, Félix María ![]() | |
dc.contributor.author | Alonso Izquierdo, Alicia ![]() | |
dc.contributor.author | Montes Burgos, Lidia Ruth ![]() | |
dc.date.accessioned | 2023-12-14T19:07:02Z | |
dc.date.available | 2023-12-14T19:07:02Z | |
dc.date.issued | 2023-11-21 | |
dc.identifier.citation | International Journal of Molecular Sciences 24(23) : (2023) // Article ID 16570 | es_ES |
dc.identifier.issn | 1422-0067 | |
dc.identifier.uri | http://hdl.handle.net/10810/63392 | |
dc.description.abstract | <i>N</i>-maleimide-derivatized phospholipids are often used to facilitate protein anchoring to membranes. In autophagy studies, this is applied to the covalent binding of Atg8, an autophagy protein, to a phosphatidylethanolamine (PE) in the nascent autophagosome. However, the question remains on how closely the <i>N</i>-maleimide PE derivative (PE-mal) mimicks the native PE in the bilayer. In the present paper, spectroscopic and calorimetric techniques have been applied to vesicles containing either PE or PE-mal (together with other phospholipids) to compare the properties of the native and derivatized forms of PE. According to differential scanning calorimetry, and to infrared spectroscopy, the presence of PE-mal did not perturb the fatty acyl chains in the bilayer. Fluorescence spectroscopy and microscopy showed that PE-mal did not alter the bilayer permeability either. However, fluorescence emission polarization of the Laurdan and DPH probes indicated an increased order, or decreased fluidity, in the bilayers containing PE-mal. In addition, the infrared spectral data from the phospholipid phosphate region revealed a PE-mal-induced conformational change in the polar heads, accompanied by increased hydration. Globally considered, the results suggest that PE-mal would be a reasonable substitute for PE in model membranes containing reconstituted proteins. | es_ES |
dc.description.sponsorship | This work was funded in part by the Spanish Ministry of Science, Innovation, and Universities (MCIU), Agencia Estatal de Investigación (AEI), Fondo Europeo de Desarrollo Regional (FEDER) (grant No. PID2021-124461NB-I00), the Basque Government (grant No. IT1625-22), Fundación Ramón Areces (CIVP20A6619), Fundación Biofísica Bizkaia and the Basque Excellence Research Centre (BERC) program of the Basque Government. E.J.G.-R. was supported by Fundación Ramón Areces. Y.R.V. was a recipient of a pre-doctoral FPU fellowship from the Spanish Ministry of Science, Innovation, and Universities (FPU18/00799). U.B. thanks the University of the Basque Country for a pre-doctoral contract. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | MDPI | es_ES |
dc.relation | info:eu-repo/grantAgreement/MCIU/PID2021-124461NB-I00 | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.subject | maleimide | es_ES |
dc.subject | membrane fluidity | es_ES |
dc.subject | differential scanning calorimetry | es_ES |
dc.subject | fluorescence | es_ES |
dc.subject | infrared spectroscopy | es_ES |
dc.subject | autophagy proteins | es_ES |
dc.title | Effects of a N-Maleimide-derivatized Phosphatidylethanolamine on the Architecture and Properties of Lipid Bilayers | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.date.updated | 2023-12-08T15:10:54Z | |
dc.rights.holder | © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). | es_ES |
dc.relation.publisherversion | https://www.mdpi.com/1422-0067/24/23/16570 | es_ES |
dc.identifier.doi | 10.3390/ijms242316570 | |
dc.departamentoes | Bioquímica y biología molecular | |
dc.departamentoeu | Biokimika eta biologia molekularra |
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Except where otherwise noted, this item's license is described as © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).