| dc.contributor.author | Gurruchaga Pereda, Juan | |
| dc.contributor.author | Martínez Martínez, Virginia  | |
| dc.contributor.author | Formoso Estensoro, Elena | |
| dc.contributor.author | Azpitarte Aguirre, Oxana | |
| dc.contributor.author | Rezabal Astigarraga, Elixabete | |
| dc.contributor.author | Lopez, Xabier | |
| dc.contributor.author | López Cortajarena, Aitziber | |
| dc.contributor.author | Salassa, Luca | |
| dc.date.accessioned | 2024-01-24T19:11:57Z | |
| dc.date.available | 2024-01-24T19:11:57Z | |
| dc.date.issued | 2021-05-07 | |
| dc.identifier.citation | Journal of Physical Chemistry Letters 12(19) : 4504–4508 ( 2021) | es_ES |
| dc.identifier.issn | 1948-7185 | |
| dc.identifier.uri | http://hdl.handle.net/10810/64312 | |
| dc.description.abstract | Our recent work demonstrates that certain flavoproteins can catalyze the redox activation of Pt(IV) prodrug complexes under light irradiation. Herein, we used site-directed mutagenesis on the mini singlet oxygen generator (mSOG) to modulate the photocatalytic activity of this flavoprotein toward two model Pt(IV) substrates. Among the prepared mutants, Q103V mSOG displayed enhanced catalytic efficiency as a result of its longer triplet excited-state lifetime. This study shows, for the first time, that protein engineering can improve the catalytic capacity of a protein toward metal-containing substrates. | es_ES |
| dc.description.sponsorship | We acknowledge the Spanish State Research Agency for Grants PID2019-109111RB-I00, PGC2018-097529-B-100, PID2019-111649RB-I00, ERACoBioTech HOMBIOCAT-PCI2018-092984, and MAT2017-83856-C3-3-P and the Spanish Multi-MetDrugs network (RED2018-102471-T) for fruitful discussion. We also thank Eusko Jaurlaritza (Grants IT1254-19 and IT912-16) and the technical and human support provided by the IZO-SGI SGIker of UPV/EHU. A.L.C. received funding from the European Research Council (ERC-CoG-648071-ProNANO). O.A. thanks UPV/EHU (PIF19/244) for financial support. This work was performed under the Severo Ochoa Centres of Excellence and Maria de Maeztu Units of Excellence Programs of the Spanish State Research Agency—Grants CEX2018-000867-S (DIPC) and MDM-2017-0720 (CIC biomaGUNE). | es_ES |
| dc.language.iso | eng | es_ES |
| dc.publisher | ACS | es_ES |
| dc.rights | info:eu-repo/semantics/openAccess | es_ES |
| dc.title | Enhancing the Photocatalytic Conversion of Pt(IV) Substrates by Flavoprotein Engineering | es_ES |
| dc.type | info:eu-repo/semantics/article | es_ES |
| dc.rights.holder | © 2021 American Chemical Society | es_ES |
| dc.relation.publisherversion | https://pubs.acs.org/doi/10.1021/acs.jpclett.1c00802 | es_ES |
| dc.identifier.doi | 10.1021/acs.jpclett.1c00802 | |
| dc.departamentoes | Polímeros y Materiales Avanzados: Física, Química y Tecnología | es_ES |
| dc.departamentoes | Química física | es_ES |
| dc.departamentoeu | Polimero eta Material Aurreratuak: Fisika, Kimika eta Teknologia | es_ES |
| dc.departamentoeu | Kimika fisikoa | es_ES |
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