dc.contributor.author | Prieto Fernández, Endika | |
dc.contributor.author | López López, Elixabet | |
dc.contributor.author | Martín Guerrero, Idoia | |
dc.contributor.author | Bárcena, Laura | |
dc.contributor.author | González López, Monika | |
dc.contributor.author | Aransay Bañares, Ana María | |
dc.contributor.author | Lozano, Juan José | |
dc.contributor.author | Benito Fernández, Francisco Javier | |
dc.contributor.author | Falcón Pérez, Juan Manuel | |
dc.contributor.author | García-Orad Carles, África | |
dc.date.accessioned | 2024-02-05T17:04:41Z | |
dc.date.available | 2024-02-05T17:04:41Z | |
dc.date.issued | 2020-07-16 | |
dc.identifier.citation | Molecular Neurobiology 57 : 4134-4142 (2020) | es_ES |
dc.identifier.issn | 1559-1182 | |
dc.identifier.issn | 0893-7648 | |
dc.identifier.uri | http://hdl.handle.net/10810/64652 | |
dc.description.abstract | The development of human brain starts in the first weeks of embryo differentiation. However, there are many relevant neurodevelopmental processes that take place after birth and during lifespan. Such a fine and changing scenario requires coordinated expression of thousands of genes to achieve the proper specialization and inter-connectivity. In this context, microRNAs (miRNAs), which can modulate mRNA stability and translation, are gaining recognition for their involvement in both brain development and neurodevelopmental disorders. Therefore, cerebrospinal fluid (CSF) miRNAs should be perfectly differentiated in relevant age periods. In this study, we aimed to highlight the biological variability of miRNA expression in the CSF throughout life, which is also crucial for biomarker discovery in CNS pathologies, especially in children, where they are desperately needed. METHODS: We analyzed the CSF microRNAome of 14 healthy children (aged 0-7.4 years) by smallRNA-Seq and compared it with previously published data in adults (N=7) and elders (N=11). RESULTS: miR-423-5p and miR-22-3p were overexpressed in the <1 and >3 year groups, respectively. Additionally, we detected 18 miRNAs that reached their highest peak of expression at different time-points during lifespan and sets of miRNAs that were exclusively expressed in a specific age group. On the contrary, miR-191-5p showed stable expression in CSF from the first year of life. CONCLUSION: Our results remark the complex differential miRNA expression profile that can be observed through life, which underlines the need for including appropriate age-matched controls when the expression of CSF miRNAs is analyzed in different pathological contexts. | es_ES |
dc.description.sponsorship | This work was supported by the Basque Government [IT989-16], the Spanish Ministry of Economy and Competitiveness MINECO [SAF2015-66312], and the Ramon Areces Foundation [FRA-17-JMF]. We thank MINECO for the TenTaCles (Spanish Excellence Network in Exosomes) and the Severo Ochoa Excellence Accreditation (SEV-2016-0644). Funding for open access charge: Severo Ochoa Excellence Accreditation (SEV-2016-0644). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Springer | es_ES |
dc.relation | info:eu-repo/grantAgreement/MINECO/SAF2015-66312 | |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.subject | cerebrospinal fluid | es_ES |
dc.subject | MicroRNAs | |
dc.subject | children | |
dc.subject | biomarkers | |
dc.subject | biological variability | |
dc.title | Variability in cerebrospinal fluid microRNAs through life | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.holder | © 2020, Springer Science Business Media, LLC, part of Springer Nature | es_ES |
dc.identifier.doi | 10.1007/s12035-020-02011-3 | |
dc.departamentoes | Genética, antropología física y fisiología animal | es_ES |
dc.departamentoeu | Genetika,antropologia fisikoa eta animalien fisiologia | es_ES |