dc.contributor.author | Unceta Zaballa, Nora | |
dc.contributor.author | Echeazarra Escudero, Leire | |
dc.contributor.author | Montaña, Mario | |
dc.contributor.author | Sallés Alvira, Joan | |
dc.contributor.author | Gómez Caballero, Alberto | |
dc.contributor.author | Goicolea Altuna, María Aranzazu | |
dc.contributor.author | Barrio Díez-Caballero, Ramón José | |
dc.date.accessioned | 2024-02-08T10:03:13Z | |
dc.date.available | 2024-02-08T10:03:13Z | |
dc.date.issued | 2012-105-10 | |
dc.identifier.citation | Journal of Pharmaceutical and Biomedical Analysis 70 : 529-533 (2012) | es_ES |
dc.identifier.issn | 0731-7085 | |
dc.identifier.issn | 1873-264X | |
dc.identifier.uri | http://hdl.handle.net/10810/65196 | |
dc.description.abstract | This work proposes a liquid chromatography-electrospray ionization ion trap mass spectrometry (LC-ESI-ITMS) method, for the quantification of sildenafil (SDF), tadalafil (TDF) and vardenafil (VDF) and their metabolites N-desmethylSDF, O-desethylSDF and N-desethylVDF, preceded by a sample preparation step based on protein and phospholipid elimination. A C8 column (150 mm × 4.6 mm, 5 μm) with ammonium formate (20mM) and acetonitrile as the mobile phase components have been used. This method has been validated, obtaining limits of quantification ranged from 1 to 2.5 ng/mL and 2 to 5 ng/g in serum and brain tissue respectively, while limits of detection ranged from 0.3 to 0.9 ng/mL in serum and 0.6 to 1.9 ng/g in brain tissue. Assay recoveries for low level QC samples were higher than 83% and the matrix effect ranged between 91% and 108% in serum and between 98% and 107% in brain tissue. The method has been applied to the quantification of these compounds in the serum and brain tissue of rats treated intraperitoneally with 10 mg/kg of SDF, TDF or VDF. | es_ES |
dc.description.sponsorship | This work was supported by the Spanish Minister of Science Innovation (Project CTQ2008-00651/BQU) and by grants from Basque Government (IT-332-10) and the Instituto de Salud Carlos III, Centro de Investigación Biomédica en Red de Salud Mental, CIBERSAM. Technical and human support provided by the Central Service of Analysis of Alava, SGIker (UPV/EHU, MICINN, GV/EJ, ESF) is gratefully acknowledged. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.relation | info:eu-repo/grantAgreement/MICINN/CTQ2008-00651/BQU | |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.subject | phosphodiesterase-5 inhibitors | es_ES |
dc.subject | metabolites | |
dc.subject | LC–ESI-ITMS | |
dc.subject | rat serum | |
dc.subject | rat brain tissue | |
dc.title | Validation of an LC-ESI-MS/MS for the quantitation of phosphodiesterase-5 inhibitors and their main metabolites in rat serum and brain tissue samples. | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.holder | © 2012 Elsevier under CC BY-NC-ND license | |
dc.relation.publisherversion | https://www.sciencedirect.com/science/article/pii/S0731708512002270 | |
dc.identifier.doi | 10.1016/j.jpba.2012.04.030 | |
dc.departamentoes | Farmacología | es_ES |
dc.departamentoes | Química analítica | es_ES |
dc.departamentoeu | Farmakologia | es_ES |
dc.departamentoeu | Kimika analitikoa | es_ES |