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dc.contributor.authorIbarguren, Maitane ORCID
dc.contributor.authorLópez Jiménez, David ORCID
dc.contributor.authorEncinar Hidalgo, José Antonio
dc.contributor.authorGonzález-Ros, Jose Manuel
dc.contributor.authorBusquets, Xavier ORCID
dc.contributor.authorEscriba, Pablo ORCID
dc.date.accessioned2024-02-08T11:28:54Z
dc.date.available2024-02-08T11:28:54Z
dc.date.issued2013-06-18
dc.identifier.citationBiochimica et Biophysica Acta - Biomembranes 1828(11) : 2553-2563 (2013)es_ES
dc.identifier.issn0005-2736
dc.identifier.issn1879-2642
dc.identifier.urihttp://hdl.handle.net/10810/65628
dc.description.abstract[EN] Cellular functions are usually associated with the activity of proteins and nucleic acids. Recent studies have shown that lipids modulate the localization and activity of key membrane-associated signal transduction proteins, thus regulating the cell's physiology. Membrane Lipid Therapy aims to reverse cell dysfunctions (i.e., diseases) by modulating the activity of membrane signaling proteins through regulation of the lipid bilayer structure. The present work shows the ability of a series of 2-hydroxyfatty acid (2OHFA) derivatives, varying in the acyl chain length and degree of unsaturation, to regulate the membrane lipid structure. These molecules have shown greater therapeutic potential than their natural non-hydroxylated counterparts. We demonstrated that both 2OHFA and natural FAs induced reorganization of lipid domains in model membranes of POPC:SM:PE:Cho, modulating the liquid-ordered/liquid-disordered structures ratio and the microdomain lipid composition. Fluorescence spectroscopy, confocal microscopy, Fourier transform infrared spectroscopy and differential detergent solubilization experiments showed a destabilization of the membranes upon addition of the 2OHFAs and FAs which correlated with the observed disordering effect. The changes produced by these synthetic fatty acids on the lipid structure may constitute part of their mechanism of action, leading to changes in the localization/activity of membrane proteins involved in signaling cascades, and therefore modulating cell responses.es_ES
dc.description.sponsorshipThis work was supported by grants from Spanish Ministerio de Economía y Competitividad, Spain (BIO2010-21132; IPT-010000-2010-16; BFU2008-00602/BMC and CSD2008-00005) Govern de les Illes Balears (Grups Competitius) and the Marathon Foundation. Ministerio de Economía y Competitividad, Spain, and the European Social Fund (PTQ-10-04214 and PTQ-09-02-02113)es_ES
dc.language.isospaes_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectfatty acidses_ES
dc.subjectlipid raftses_ES
dc.subjectmembrane lipidses_ES
dc.subjectmembrane structurees_ES
dc.subjectmembrane fluidityes_ES
dc.subjectmembrane lipid therapyes_ES
dc.titlePartitioning of liquid-ordered/liquid-disordered membrane microdomains induced by the fluidifying effect of 2-hydroxylated fatty acid derivativeses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.holder© 2013 Elsevier under CC BY-NC-ND license*
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0005273613002034es_ES
dc.identifier.doi10.1016/j.bbamem.2013.06.014
dc.departamentoesBioquímica y biología moleculares_ES
dc.departamentoeuBiokimika eta biologia molekularraes_ES


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© 2013 Elsevier under CC BY-NC-ND license
Except where otherwise noted, this item's license is described as © 2013 Elsevier under CC BY-NC-ND license