CircRNAome of Childhood Acute Lymphoblastic Leukemia: Deciphering Subtype-Specific Expression Profiles and Involvement in TCF3::PBX1 ALL
dc.contributor.author | Gutiérrez Camino, Ángela ![]() | |
dc.contributor.author | Caron, Maxime | |
dc.contributor.author | Richer, Chantal | |
dc.contributor.author | Fuchs, Claire | |
dc.contributor.author | Illarregi, Unai | |
dc.contributor.author | Poncelet, Lucas | |
dc.contributor.author | St-Onge, Pascal | |
dc.contributor.author | Bataille, Alain R. | |
dc.contributor.author | Tremblay-Dauphinais, Pascal | |
dc.contributor.author | López López, Elixabet ![]() | |
dc.contributor.author | Camos, Mireia | |
dc.contributor.author | Ramírez Orellana, Manuel | |
dc.contributor.author | Astigarraga Aguirre, María Iciar | |
dc.contributor.author | Lécuyer, Éric | |
dc.contributor.author | Bourque, Guillaume | |
dc.contributor.author | Martín Guerrero, Idoia | |
dc.contributor.author | Sinnett, Daniel | |
dc.date.accessioned | 2024-04-10T16:02:11Z | |
dc.date.available | 2024-04-10T16:02:11Z | |
dc.date.issued | 2024-01-25 | |
dc.identifier.citation | International Journal of Molecular Sciences 25(3) : (2024) // Article ID 1477 | es_ES |
dc.identifier.issn | 1422-0067 | |
dc.identifier.uri | http://hdl.handle.net/10810/66607 | |
dc.description.abstract | Childhood B-cell acute lymphoblastic leukemia (B-ALL) is a heterogeneous disease comprising multiple molecular subgroups with subtype-specific expression profiles. Recently, a new type of ncRNA, termed circular RNA (circRNA), has emerged as a promising biomarker in cancer, but little is known about their role in childhood B-ALL. Here, through RNA-seq analysis in 105 childhood B-ALL patients comprising six genetic subtypes and seven B-cell controls from two independent cohorts we demonstrated that circRNAs properly stratified B-ALL subtypes. By differential expression analysis of each subtype vs. controls, 156 overexpressed and 134 underexpressed circRNAs were identified consistently in at least one subtype, most of them with subtype-specific expression. TCF3::PBX1 subtype was the one with the highest number of unique and overexpressed circRNAs, and the circRNA signature could effectively discriminate new patients with TCF3::PBX1 subtype from others. Our results indicated that NUDT21, an RNA-binding protein (RBP) involved in circRNA biogenesis, may contribute to this circRNA enrichment in TCF3::PBX1 ALL. Further functional characterization using the CRISPR-Cas13d system demonstrated that circBARD1, overexpressed in TCF3::PBX1 patients and regulated by NUDT21, might be involved in leukemogenesis through the activation of p38 via hsa-miR-153-5p. Our results suggest that circRNAs could play a role in the pathogenesis of childhood B-ALL. | es_ES |
dc.description.sponsorship | This work was supported by the Canadian Cancer Society Research Institute (CCSRI) (705469) and the Fundación Mutua Madrileña (AP171202019). AGC was supported by a post-doctoral fellowship from the Canadian Institute of Health Research (CIHR) (201910MFE-430442-73764) and Fundación Vasca de Innovación e Investigación Sanitarias (BIO20/CI/016/BIOEF). MC received a PhD studentship from the FRQS. UI was supported with a pre-doctoral contract from UPV/EHU and an EACR-Worldwide Cancer Research funded Travel Fellowship (EACRref:#795). We are indebted to the “Biobanc de l’Hospital Infantil Sant Joan de Déu per a la Investigació” integrated in the Spanish Biobank Network of ISCIII for the sample and data procurement. GB obtained funding from CIHR grant 245250 and is funded by the FRQS. D.S. holds the FKV chair in pediatric oncogenomics. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | MDPI | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/es/ | |
dc.subject | acute lymphoblastic leukemia | es_ES |
dc.subject | childhood B-ALL | es_ES |
dc.subject | circular RNA | es_ES |
dc.subject | TCF3::PBX1 | es_ES |
dc.subject | RNA-binding protein | es_ES |
dc.subject | NUDT21 | es_ES |
dc.title | CircRNAome of Childhood Acute Lymphoblastic Leukemia: Deciphering Subtype-Specific Expression Profiles and Involvement in TCF3::PBX1 ALL | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.date.updated | 2024-02-09T15:06:55Z | |
dc.rights.holder | © 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/ 4.0/). | es_ES |
dc.relation.publisherversion | https://www.mdpi.com/1422-0067/25/3/1477 | es_ES |
dc.identifier.doi | 10.3390/ijms25031477 | |
dc.departamentoes | Bioquímica y biología molecular | |
dc.departamentoes | Genética, antropología física y fisiología animal | |
dc.departamentoeu | Biokimika eta biologia molekularra | |
dc.departamentoeu | Genetika,antropologia fisikoa eta animalien fisiologia |
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Except where otherwise noted, this item's license is described as © 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/ 4.0/).