dc.contributor.author | Casas, Jesús | |
dc.contributor.author | Ibarguren, Maitane ![ORCID](/themes/Mirage2//images/orcid_16x16.png) | |
dc.contributor.author | Álvarez, Rafael | |
dc.contributor.author | Terés, Silvia | |
dc.contributor.author | LLadó, Victoria | |
dc.contributor.author | Piotto, Stefano | |
dc.contributor.author | Concilio, Simona | |
dc.contributor.author | Busquets, Xavier ![ORCID](/themes/Mirage2//images/orcid_16x16.png) | |
dc.contributor.author | López Jiménez, David ![ORCID](/themes/Mirage2//images/orcid_16x16.png) | |
dc.contributor.author | Escriba, Pablo V. | |
dc.date.accessioned | 2024-04-12T18:34:27Z | |
dc.date.available | 2024-04-12T18:34:27Z | |
dc.date.issued | 2017-07-03 | |
dc.identifier.citation | Biochimica et Biophysica Acta - Biomembranes 1859(9) : 1526-1535 (2017) | es_ES |
dc.identifier.issn | 0005-2736 | |
dc.identifier.uri | http://hdl.handle.net/10810/66655 | |
dc.description.abstract | [EN] G proteins often bear myristoyl, palmitoyl and isoprenyl moieties, which favor their
association with the membrane and their accumulation in G Protein Coupled Receptor-rich
microdomains. These lipids influence the biophysical properties of membranes and thereby
modulate G protein binding to bilayers. In this context, we showed here that geranylgeraniol, but
neither myristate nor palmitate, increased the inverted hexagonal (HII) phase propensity of
phosphatidylethanolamine-containing membranes. While myristate and palmitate preferentially
associated with phosphatidylcholine membranes, geranylgeraniol favored nonlamellar-prone
membranes. In addition, Gi1 monomers had a higher affinity for lamellar phases, while G and
G showed a marked preference for nonlamellar prone membranes. Moreover, geranylgeraniol
enhanced the binding of G protein dimers and trimers to phosphatidylethanolamine-containing
membranes, yet it decreased that of monomers. By contrast, both myristate and palmitate
increased the Gi1 preference for lamellar membranes. Palmitoylation reinforced the binding of
the monomer to PC membranes and myristoylation decreased its binding to PE-enriched bilayer.
Finally, binding of dimers and trimers to lamellar-prone membranes was decreased by palmitate
and myristate, but it was increased in nonlamellar-prone bilayers. These results demonstrate that
co/post-translational G protein lipid modifications regulate the membrane lipid structure and that
they influence the physico-chemical properties of membranes, which in part explains why G
protein subunits sort to different plasma membrane domains. | es_ES |
dc.description.sponsorship | This study was supported by the Spanish Ministerio de Economía y Competitividad grant BIO2010-21132, BIO2013-49006-C2-1-R, RTC-2015-3542-1 and RTC-2015-4094-1, cofinanced by FEDER funds from the EU (“Una manera de hacer Europa”), by the Govern de les Illes Balears (Grups competitius and Research Excellent Grant) and the Marathon Foundation. JC and RA were supported by predoctoral fellowships from the Ministerio de Ciencia e Innovación and from the Ministerio de Educación, Cultura y Deporte, respectively. ST, MI and DJL hold a Torres-Quevedo contract from the Spanish Ministerio de Economía y Competitividad. VL is supported by a postdoctoral contract from the Asociación Española Contra el Cáncer. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | G-proteins | es_ES |
dc.subject | membranes | es_ES |
dc.subject | palmitoylation | es_ES |
dc.subject | myristoylation | es_ES |
dc.subject | cell signaling | es_ES |
dc.subject | isoprenoids | es_ES |
dc.title | G protein-membrane interactions II: Effect of G Protein-linked Lipids on Membrane Structure and G Protein-membrane Interactions | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.holder | © 2017 Elsevier under CC BY-NC-ND license | es_ES |
dc.relation.publisherversion | https://www.sciencedirect.com/science/article/pii/S0005273617301219?via%3Dihub | es_ES |
dc.identifier.doi | 10.1016/j.bbamem.2017.04.005 | |
dc.departamentoes | Bioquímica y biología molecular | es_ES |
dc.departamentoeu | Biokimika eta biologia molekularra | es_ES |