dc.contributor.author | Musatadi Larrucea, Mikel | |
dc.contributor.author | Álvarez Mora, Iker | |
dc.contributor.author | Baciero Hernández, Inés | |
dc.contributor.author | Anakabe Iturriaga, Eneritz | |
dc.contributor.author | Olivares Zabalandikoetxea, Maitane | |
dc.contributor.author | Extebarria Loizate, Nestor | |
dc.contributor.author | Zuloaga Zubieta, Olatz | |
dc.date.accessioned | 2024-04-16T15:14:40Z | |
dc.date.available | 2024-04-16T15:14:40Z | |
dc.date.issued | 2024-01-18 | |
dc.identifier.citation | Talanta 271 : (2024) // Article ID 125698 | es_ES |
dc.identifier.issn | 0039-9140 | |
dc.identifier.issn | 1873-3573 | |
dc.identifier.uri | http://hdl.handle.net/10810/66709 | |
dc.description.abstract | Persistent, mobile and toxic substances have drawn attention nowadays due to their particular properties, but they are overlooked in human monitorization works, limiting the knowledge of the human exposome. In that sense, human urine is an interesting matrix since not only parent compounds are eliminated, but also their phase II metabolites that could act as biomarkers. In this work, 11 sample preparation procedures involving preconcentration were tested to ensure maximum analytical coverage in human urine using mixed-mode liquid chromatography coupled with high-resolution tandem mass spectrometry. The optimized procedure consisted of a combination of solid-phase extraction and salt-assisted liquid-liquid extraction and it was employed for suspect screening. Additionally, a non-discriminatory dilute-and-shoot approach was also evaluated. After evaluating the workflow in terms of limits of identification and type II errors (i.e., false negatives), a pooled urine sample was analysed. From a list of 1450 suspects and in-silico simulated 1568 phase II metabolites (i.e. sulphates, glucuronides, and glycines), 44 and 14 substances were annotated, respectively. Most of the screened suspects were diverse industrial chemicals, but biocides, natural products and pharmaceuticals were also detected. Lastly, the complementarity of the sample preparation procedures, columns, and analysis conditions was assessed. As a result, dilute-and-shoot and the Acclaim Trinity P1 column at pH =3 (positive ionization) and pH =7 (negative ionization) allowed the maximum coverage since almost 70 % of the total suspects could be screened using those conditions. | es_ES |
dc.description.sponsorship | The authors acknowledge financial support from the State Research Agency of the Ministry of Science and Innovation, Government of Spain (project PID2020-117686RB-C31) and the Basque Government as a consolidated group of the Basque Research System (IT-1446-22). M. Musatadi and I. Baciero-Hernandez also acknowledge the Basque Government for their predoctoral fellowship. Lastly, I. Alvarez-Mora acknowledges the Basque Government for his postdoctoral fellowship as well. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elservier | es_ES |
dc.relation | info:eu-repo/grantAgreement/MCIN/PID2020-117686RB-C31 | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/es/ | * |
dc.subject | Persistent mobile and toxic substances | es_ES |
dc.subject | exposome | es_ES |
dc.subject | phase II metabolites | es_ES |
dc.subject | mix-mode liquid chromatography | es_ES |
dc.subject | high resolution mass spectrometry | es_ES |
dc.subject | suspect screening | es_ES |
dc.title | Sample preparation for suspect screening of persistent, mobile and toxic substances and their phase II metabolites in human urine by mixed-mode liquid chromatography | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.holder | © 2024 The Author(s). Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license | es_ES |
dc.relation.publisherversion | https://www.sciencedirect.com/science/article/pii/S0039914024000778 | es_ES |
dc.identifier.doi | 10.1016/j.talanta.2024.125698 | |
dc.departamentoes | Química analítica | es_ES |
dc.departamentoeu | Kimika analitikoa | es_ES |