dc.contributor.author | Agirrezabala Urkia, Ziortza | |
dc.contributor.author | Guruceaga Sierra, Xabier | |
dc.contributor.author | Martín Vicente, Adele | |
dc.contributor.author | Otamendi Elizalde, Ainara | |
dc.contributor.author | Fagoaga Izquierdo, Ane | |
dc.contributor.author | Fortwendel, Jarrod R. | |
dc.contributor.author | Espeso, Eduardo A. | |
dc.contributor.author | Echeveste Juárez, Oier ![ORCID](/themes/Mirage2//images/orcid_16x16.png) | |
dc.date.accessioned | 2024-05-03T17:21:41Z | |
dc.date.available | 2024-05-03T17:21:41Z | |
dc.date.issued | 2023-11 | |
dc.identifier.citation | mBio 14(6) : (2023) // Article ID e02452-23 | es_ES |
dc.identifier.issn | 2150-7511 | |
dc.identifier.issn | 2161-2129 | |
dc.identifier.uri | http://hdl.handle.net/10810/67467 | |
dc.description.abstract | Asexual spores are the main vehicle used by fungi to disperse to new niches. The Eurotiomycete Aspergillus nidulans is the main reference for the study of the genetic/molecular control of asexual development. In this species, Flb proteins control the expression of the master gene brlA, and thus, loss-of-function mutations in flb (upstream developmental activation [UDA]) genes block brlA transcription and, consequently, the production of conidiophores, the structures bearing asexual spores known as conidia. However, the aconidial phenotype of specific flb mutants, such as that of the ΔflbB strain, is reverted under salt-stress conditions. Previously, we generated a collection of second-site mutants of ΔflbB unable to conidiate on culture medium supplemented with NaH2PO4 (0.65 M). Here, we identified a Gly347Stop mutation within flpA as responsible for the FLIP57 phenotype and characterized the role of the putative cyclin FlpA and the remaining putative components of the C-terminal domain kinase-1 (CTDK-1) complex in A. nidulans and Aspergillus fumigatus. FlpA, Stk47, and FlpB are necessary (i) for timely germination, (ii) in the transition from metulae to phialides (the cells generating conidia) during conidiophore development, and (iii) for the development of sexual structures (cleistothecia) in A. nidulans. The three proteins are nuclear, and the nucleoplasmic localization of Stk47 depends on the activity of FlpA, which correlates with the retention of Stk47 by FlpA in pull-down assays. Overall, this work links the putative CTDK-1 complex of aspergilli with growth and developmental control. Identification of a mutation in flpA as inhibitor of conidiation in A. nidulans and functional characterization of FlpA, Stk47 and FlpB as putative members of the C-terminal domain kinase complex CTDK-1 in A. nidulans and A. fumigatus. | es_ES |
dc.description.sponsorship | Work at O.E.'s lab has been supported by Universidad del País Vasco (UPV)/Euskal Herriko Unibertsitatea (EHU) (GIU19/014) and the Basque Government (PIBA-PUE PIBA_2020_1_0032; Elkartek KK-2021/43 and KK-2022/00107; and GIC IT1662-22). Work at CIB Margarita Salas-CSIC has been supported by Ministerio de Ciencia, Innovaciόn y Universidades/Spanish Agencia Estatal de Investigación (RTI2018-094263-B-100, to E.A.E.). A.O. held a Margarita Salas grant (MARSA21/69), funded by Next-Generation EU, at the UPV/EHU. A.F. was a degree student with a collaboration grant by the Spanish Ministry of Education (21/19070). Z.A. was a master thesis student at O.E.'s lab, held an Ikertalent Fellowship funded by the Basque Government (PIF21/003) at the Basque Culinary Center, and is now a PhD student at O.E.'s lab with funds of the KK-2022/00107 project. Work at J.R.F.'s lab has been supported by National Institutes of Health grants R01-AI158442 and R01-AI143197. The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | American Society of Microbiology | es_ES |
dc.relation | info:eu-repo/grantAgreement/MICIU/RTI2018-094263-B-100 | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.subject | filamentous fungi | es_ES |
dc.subject | Aspergillus nidulans | es_ES |
dc.subject | Aspergillus fumigatus | es_ES |
dc.subject | vegetative growth | es_ES |
dc.subject | asexual development | es_ES |
dc.subject | conidiation | es_ES |
dc.subject | sexual development | es_ES |
dc.subject | stress response | es_ES |
dc.subject | cyclin | es_ES |
dc.subject | kinase | es_ES |
dc.subject | CTDK-1 | es_ES |
dc.subject | RNA polymerase II | es_ES |
dc.title | Identification and functional characterization of the putative members of the CTDK-1 kinase complex as regulators of growth and development in Aspergillus nidulans and Aspergillus fumigatus | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.holder | © 2023 Agirrezabala et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. | es_ES |
dc.rights.holder | Atribución 3.0 España | * |
dc.relation.publisherversion | https://journals.asm.org/doi/10.1128/mbio.02452-23 | es_ES |
dc.identifier.doi | 10.1128/mbio.02452 | |
dc.departamentoes | Química aplicada | es_ES |
dc.departamentoeu | Kimika aplikatua | es_ES |