Engineered metal-loaded biohybrids to promote the attachment and electron-shuttling between enzymes and carbon electrodes
dc.contributor.author | Rodríguez Abetxuko, Andoni | |
dc.contributor.author | Romero Ben, Elena | |
dc.contributor.author | Ontoria Jimenez, Aitor | |
dc.contributor.author | Heredero Iborra, Marcos | |
dc.contributor.author | Martín García, Beatriz | |
dc.contributor.author | Kumar, Krishan | |
dc.contributor.author | Martín Saldaña, Sergio | |
dc.contributor.author | Conzuelo, Felipe | |
dc.contributor.author | Beloqui Elizazu, Ana | |
dc.date.accessioned | 2024-05-20T17:25:17Z | |
dc.date.available | 2024-05-20T17:25:17Z | |
dc.date.issued | 2024-05 | |
dc.identifier.citation | Advanced Functional Materials 34(18) : (2024) // Article ID 2400479 | es_ES |
dc.identifier.issn | 1616-301X | |
dc.identifier.issn | 1616-3028 | |
dc.identifier.uri | http://hdl.handle.net/10810/68055 | |
dc.description.abstract | The inorganic content and the catalytic performance pose metal-loaded enzyme nanoflowers as promising candidates for developing bioelectrodes capable of functioning without the external addition of a redox mediator. However, these protein-inorganic hybrids have yet to be successfully applied in combination with electrode materials. Herein, the synthesis procedure of these bionanomaterials is reproposed to precisely control the morphology, composition, and performance of this particular protein-mineral hybrid, formed by glucose oxidase and cobalt phosphate. This approach aims to enhance the adherence and electron mobility between the enzyme and a carbon electrode. The strategy relies on dressing the protein in a tailored thin nanogel with multivalent chemical motifs. The functional groups of the polymer facilitate the fast protein sequence-independent biomineralization. Furthermore, the engineered enzymes enable the fabrication of robust cobalt-loaded enzyme inorganic hybrids with exceptional protein loads, exceeding 90% immobilization yields. Notably, these engineered biohybrids can be readily deposited onto flat electrode surfaces without requiring chemical pre-treatment. The resulting bioelectrodes are robust and exhibit electrochemical responses even without the addition of a redox mediator, suggesting that cobalt complexes promote electron wiring between the active site of the enzyme and the electrode. | es_ES |
dc.description.sponsorship | A.R.A. and E.R.B. contributed equally to this work. A.R.A. and E.R.B. shared co-authorship to this article. We thank Prof. Francesca Paradisi and Dr. Ana I. Benitez-Mateos for sharing the HeWT enzyme. A.B. gratefully acknowledges the financial support from the Spanish Research Agency (AEI) for the financial support (PID2022-142128NB-I00 funded by MCIN/ AEI/10.13039/501100011033/ and by the “European Union NextGenerationEU/PRTR”; RYC2018-025923-I from RyC program – MCIN/ AEI /10.13039/501100011033 and FSE “invierte en tu futuro”), BBVA Foundation – IN[21]_CBB_QUI_0086, and UPV/EHU- GIU21-033). K.K. thanks JdC Grant FJC2021-047607-I funded by MCIN/AEI/ 10.13039/501100011033. F.C. acknowledges FCT – Fundação para a Ciência e a Tecnologia, I.P. for the researcher contract 2022.05842.CEECIND/CP1725/CT0001 under the Scientific Employment Stimulus – Individual Call 2022. B.M.-G. thanks to support from the “Ramón y Cajal” Program by the Spanish MCIN/AEI (grant no. RYC2021-034836-I) and IKERBASQUE HYMNOS project | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Wiley | es_ES |
dc.relation | info:eu-repo/grantAgreement/MICINN/PID2022-142128NB-I00 | es_ES |
dc.relation | info:eu-repo/grantAgreement/MICIU/RYC2018-025923-I | es_ES |
dc.relation | info:eu-repo/grantAgreement/MICINN/FJC2021-047607-I | es_ES |
dc.relation | info:eu-repo/grantAgreement/MICINN/RYC2021-034836-I | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by-nc/3.0/es/ | * |
dc.subject | bioelectrochemistry | es_ES |
dc.subject | electron shuttle | es_ES |
dc.subject | protein hybrids | es_ES |
dc.subject | protein nanoflowers | es_ES |
dc.subject | protein-polymer hybrids | es_ES |
dc.title | Engineered metal-loaded biohybrids to promote the attachment and electron-shuttling between enzymes and carbon electrodes | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.holder | © 2024 The Authors. Advanced Functional Materials published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. | es_ES |
dc.rights.holder | Atribución-NoComercial 3.0 España | * |
dc.relation.publisherversion | https://onlinelibrary.wiley.com/doi/full/10.1002/adfm.202400479 | es_ES |
dc.identifier.doi | 10.1002/adfm.202400479 | |
dc.departamentoes | Química aplicada | es_ES |
dc.departamentoeu | Kimika aplikatua | es_ES |
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Except where otherwise noted, this item's license is described as © 2024 The Authors. Advanced Functional Materials published by Wiley-VCH GmbH.
This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.