dc.contributor.author | Areitio Beramendi, Maialen | |
dc.contributor.author | Antoran Díaz, Aitziber | |
dc.contributor.author | Rodríguez Ereñaga, Oier | |
dc.contributor.author | Aparicio Fernández, Leire | |
dc.contributor.author | Martín Souto, Leire | |
dc.contributor.author | Buldain Garriz, Idoia | |
dc.contributor.author | Zaldibar Aramburu, Beñat | |
dc.contributor.author | Ruiz Gaitán, Alba | |
dc.contributor.author | Pemán, Javier | |
dc.contributor.author | Rementeria Ruiz, Aitor Domingo | |
dc.contributor.author | Ramírez Garcia, Andoni | |
dc.date.accessioned | 2024-07-04T16:34:52Z | |
dc.date.available | 2024-07-04T16:34:52Z | |
dc.date.issued | 2024-04 | |
dc.identifier.citation | Journal of Proteome Research 23(5) : 1634-1648 (2024) | es_ES |
dc.identifier.issn | 1535-3893 | |
dc.identifier.issn | 1535-3907 | |
dc.identifier.uri | http://hdl.handle.net/10810/68778 | |
dc.description.abstract | The delay in making a correct diagnosis of Candida auris causes concern in the healthcare system setting, and immunoproteomics studies are important to identify immunoreactive proteins for new diagnostic strategies. In this study, immunocompetent murine systemic infections caused by non-aggregative and aggregative phenotypes of C. auris and by Candida albicans and Candida haemulonii were carried out, and the obtained sera were used to study their immunoreactivity against C. auris proteins. The results showed higher virulence, in terms of infection signs, weight loss, and histopathological damage, of the non-aggregative isolate. Moreover, C. auris was less virulent than C. albicans but more than C. haemulonii. Regarding the immunoproteomics study, 13 spots recognized by sera from mice infected with both C. auris phenotypes and analyzed by mass spectrometry corresponded to enolase, phosphoglycerate kinase, glyceraldehyde-3-phosphate dehydrogenase, and phosphoglycerate mutase. These four proteins were also recognized by sera obtained from human patients with disseminated C. auris infection but not by sera obtained from mice infected with C. albicans or Aspergillus fumigatus. Spot identification data are available via ProteomeXchange with the identifier PXD049077. In conclusion, this study showed that the identified proteins could be potential candidates to be studied as new diagnostic or even therapeutic targets for C. auris. | es_ES |
dc.description.sponsorship | This research was funded by the Basque Government, grant number numbers IT1362-19 and IT1657-22. M.A., O.R.-E., and L.M.-S. have received a predoctoral grant from the Basque Government and L.A.-F. from the University of the Basque Country (UPV/EHU). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | ACS | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.subject | Candida auris | es_ES |
dc.subject | antigen | es_ES |
dc.subject | electrophoresis | es_ES |
dc.subject | proteomic | es_ES |
dc.subject | WB | es_ES |
dc.subject | mass spectrometry | es_ES |
dc.title | Identification of the Most Immunoreactive Antigens of Candida auris to IgGs from Systemic Infections in Mice | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.holder | © 2024 The Authors. Published by American Chemical Society. This publication is licensed under
CC-BY 4.0. | es_ES |
dc.rights.holder | Atribución 3.0 España | * |
dc.relation.publisherversion | https://pubs.acs.org/doi/10.1021/acs.jproteome.3c00752 | es_ES |
dc.identifier.doi | 10.1021/acs.jproteome.3c00752 | |
dc.departamentoes | Inmunología, microbiología y parasitología | es_ES |
dc.departamentoes | Zoología y biología celular animal | es_ES |
dc.departamentoeu | Immunologia, mikrobiologia eta parasitologia | es_ES |
dc.departamentoeu | Zoologia eta animalia zelulen biologia | es_ES |