BackExaminar Artículos por departamento (eus.) "Biokimika eta biologia molekularra"
Now showing items 1-20 of 310
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A case for the importance of following antibiotic resistant bacteria throughout the soil food web
(Wiley, 2023-12)It is necessary to complement next-generation sequencing data on the soil resistome with theoretical knowledge provided by ecological studies regarding the spread of antibiotic resistant bacteria (ARB) in the abiotic and, ... -
A cellular reporter to evaluate CRM1 nuclear export activity: functional analysis of the cancer-related mutant E571K
García Santisteban, Iraia
; ; ; Urbaneja Arrue, María Ángeles ; ; Bañuelos Rodríguez, Sonia ; Rodríguez Pérez, José Antonio (Springer Nature Switzerland AG, 2016-12)
The exportin CRM1 binds nuclear export signals (NESs), and mediates active transport of NES-bearing proteins from the nucleus to the cytoplasm. Structural and biochemical analyses have uncovered the molecular mechanisms ... -
A Computational Module Assembled from Different Protease Family Motifs Identifies PI PLC from Bacillus cereus as a Putative Prolyl Peptidase with a Serine Protease Scaffold
; ; ; ; ; ; ; Goñi Urcelay, Félix María; (Public Library Science, 2013-08)
Proteolytic enzymes have evolved several mechanisms to cleave peptide bonds. These distinct types have been systematically categorized in the MEROPS database. While a BLAST search on these proteases identifies homologous ... -
A fluorogenic cyclic peptide for imaging and quantification of drug-induced apoptosis
Barth, Nicole D.; Subirós Funosas, Ramón; Mendive Tapia, Lorena; Duffin, Rodger; Shields, Mario A.; Cartwright, Jennifer A.; Troeira Henriques, Sónia; Sot, Jesús; Goñi Urcelay, Félix María; Lavilla, Rodolfo; Marwick, John A.; Vermeren, Sonja; Rossi, Adriano G.; Egeblad, Mikala; Dransfield, Ian; Vendrell, Marc (Nature, 2020-08-12)
Programmed cell death or apoptosis is a central biological process that is dysregulated in many diseases, including inflammatory conditions and cancer. The detection and quantification of apoptotic cells in vivo is hampered ... -
A functional variant that affects exon-skipping and protein expression of SP140 as genetic mechanism predisposing to multiple sclerosis
(Oxford University Pres, 2015-10-01)Several variants in strong linkage disequilibrium (LD) at the SP140 locus have been associated with multiple sclerosis (MS), Crohn's disease (CD) and chronic lymphocytic leukemia (CLL). To determine the causal polymorphism, ... -
A global survey of CRM1-dependent nuclear export sequences in the human deubiquitinase family
García Santisteban, Iraia; Bañuelos Rodríguez, Sonia ; Rodríguez Pérez, José Antonio (Portland Press LTD, 2012-01-01)
The mechanisms that regulate the nucleocytoplasmic localization of human deubiquitinases remain largely unknown. The nuclear export receptor CRM1 binds to specific amino acid motifs termed NESs (nuclear export sequences). ... -
A key function for microtubule-associated-protein 6 in activity-dependent stabilisation of actin filaments in dendritic spines
; ; Martínez Hernández, José; ; ; ; ; ; ; ; ; ; ; ; ; ; ; (Nature Publishing, 2018-09-17)
Emerging evidence indicates that microtubule-associated proteins (MAPs) are implicated in synaptic function; in particular, mice deficient for MAP6 exhibit striking deficits in plasticity and cognition. How MAP6 connects ... -
A long non-coding RNA that harbors a SNP associated with type 2 diabetes regulates the expression of TGM2 gene in pancreatic beta cells
González Moro, Itziar; ; Sebastián de la Cruz, Maialen ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; (Frontiers Media, 2023-02)
IntroductionMost of the disease-associated single nucleotide polymorphisms (SNPs) lie in non- coding regions of the human genome. Many of these variants have been predicted to impact the expression and function of long ... -
A novel RT-QPCR-based assay for the relative quantification of residue specific m6A RNA methylation
(Nature Publishing Group, 2019-12)N6-methyladenosine (m6A) is the most common and abundant RNA modification. Recent studies have shown its importance in the regulation of several biological processes, including the immune response, and different approaches ... -
A Proteomic Approach for Systematic Mapping of Substrates of Human Deubiquitinating Enzymes
Ramírez Sánchez, Juan Manuel; Prieto Agujeta, Gorka ; ; ; ; Alduntzin Egizurain, Unai ; Osinalde Moraleja, Nerea ; ; ; ; Rodríguez Pérez, José Antonio ; ; ; ; ; Arizmendi Bastarrika, Jesús María ; Mayor Martínez, Ugo (MDPI, 2021-05-03)
The human genome contains nearly 100 deubiquitinating enzymes (DUBs) responsible for removing ubiquitin moieties from a large variety of substrates. Which DUBs are responsible for targeting which substrates remain mostly ... -
A Systematic Approach to Assess the Activity and Classification of PCSK9 Variants
(MDPI, 2021-12-18)Background: Gain of function (GOF) mutations of PCSK9 cause autosomal dominant familial hypercholesterolemia as they reduce the abundance of LDL receptor (LDLR) more efficiently than wild-type PCSK9. In contrast, PCSK9 ... -
Aberrant Synaptic PTEN in Symptomatic Alzheimer's Patients May Link Synaptic Depression to Network Failure
(Frontiers Media, 2021-05-11)In Alzheimer's disease (AD), Amyloid beta (A beta) impairs synaptic function by inhibiting long-term potentiation (LTP), and by facilitating long-term depression (LTD). There is now evidence from AD models that A beta ... -
Accumulated Bending Energy Elicits Neutral Sphingomyelinase Activity in Human Red Blood Cells
López Jiménez, David; ; ; ; ; ; ; ; Goñi Urcelay, Félix María ; Alonso Izquierdo, Alicia (Elservier, 2012-05-02)
[EN] We propose that accumulated membrane bending energy elicits a neutral sphingomyelinase (SMase) activity in human erythrocytes. Membrane bending was achieved by osmotic or chemical processes, and SMase activity was ... -
Actin- regulated Siglec- 1 nanoclustering influences HIV-1 capture and virus- containing compartment formation in dendritic cells
(eLife Sciences, 2023-03)The immunoglobulin-like lectin receptor CD169 (Siglec-1) mediates the capture of HIV- 1 by activated dendritic cells (DCs) through binding to sialylated ligands. These interactions result in a more efficient virus capture ... -
Activation of the DnaK-ClpB Complex is Regulated by the Properties of the Bound Substrate
; ; ; Moro Pérez, Fernando; Muga Villate, Arturo (Nature Publishing, 2018-04-11)
The chaperone ClpB in bacteria is responsible for the reactivation of aggregated proteins in collaboration with the DnaK system. Association of these chaperones at the aggregate surface stimulates ATP hydrolysis, which ... -
Activity-associated effect of LDL receptor missense variants located in the cysteine-rich repeats
(Elsevier, 2015-02)Background: The LDL receptor (LDLR) is a Class I transmembrane protein critical for the clearance of cholesterol-containing lipoprotein particles. The N-terminal domain of the LDLR harbours the ligand-binding domain ... -
Adenylate Cyclase Toxin promotes bacterial internalisation into non phagocytic cells
; ; ; ; González Bullón, David; Arluzea de Jauregizar, Jon Andoni ; Goñi Urcelay, Félix María ; Arechaga Martínez, Juan Miguel ; (Nature Publishing Group, 2015-09-08)
Bordetella pertussis causes whooping cough, a respiratory infectious disease that is the fifth largest cause of vaccine-preventable death in infants. Though historically considered an extracellular pathogen, this bacterium ... -
Adenylate Cyclase Toxin Promotes Internalisation of Integrins and Raft Components and Decreases Macrophage Adhesion Capacity
(Public Library of Science, 2011-02-23)Bordetella pertussis, the bacterium that causes whooping cough, secretes an adenylate cyclase toxin (ACT) that must be post-translationally palmitoylated in the bacterium cytosol to be active. The toxin targets phagocytes ... -
Advantages and Versatility of Fluorescence-Based Methodology to Characterize the Functionality of LDLR and Class Mutation Assignment
(Public Library Science, 2014-11-11)Familial hypercholesterolemia (FH) is a common autosomal codominant disease with a frequency of 1:500 individuals in its heterozygous form. The genetic basis of FH is most commonly mutations within the LDLR gene. Assessing ... -
Affinity for the Interface Underpins Potency of Antibodies Operating In Membrane Environments
; ; ; ; ; ; Sánchez Eugenia, Rubén; ; ; ; ; ; ; ; ; ; ; ; (Cell Press, 2020-08-18)
The contribution of membrane interfacial interactions to recognition of membrane-embedded antigens by antibodies is currently unclear. This report demonstrates the optimization of this type of antibodies via chemical ...