dc.contributor.advisor | Reichardt, Niels Christian | |
dc.contributor.advisor | Jiménez Barbero, Jesús | |
dc.contributor.advisor | Palomo Nicolau, Claudio | |
dc.contributor.author | Cioce, Anna | |
dc.date.accessioned | 2020-03-27T08:11:15Z | |
dc.date.available | 2020-03-27T08:11:15Z | |
dc.date.issued | 2019-02-01 | |
dc.date.submitted | 2019-02-01 | |
dc.identifier.uri | http://hdl.handle.net/10810/42433 | |
dc.description | 209 p. | es_ES |
dc.description.abstract | Carbohydrate microarrays are well established tools for high-throughput analysis of carbohydrate-protein interactions and for screening the specificity of carbohydrate processing enzymes, In addition, a few groups have also explored the on-chip synthesis of ligands. The use of small amounts of substrates and the absence of long purification step make the microarray a time-saving approach for the parallel synthesis of hundred scaffolds.We developed a versatile platform to quickly synthesize significant number of glycomimetics via chemoenzymatic protocols and test them as potential ligands for C-type lectin receptors (CLRs). The transparent and the conductive hydrophobic-ITO surface allows to perform binding studies with labeled lectins by fluorescent spectroscopy as well as to estimate conversion rates of the on-chip reactions for every printed ligand by MALDI-TOF MS. | es_ES |
dc.description.sponsorship | CIC biomaGUNE | es_ES |
dc.language.iso | eng | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.subject | bioquímica inmunológica | es_ES |
dc.subject | polisacáridos | es_ES |
dc.subject | química de los hidratos de carbono | es_ES |
dc.subject | immunochemistry | es_ES |
dc.subject | polysaccharides | es_ES |
dc.subject | carbohydrate chemistry | es_ES |
dc.title | Synthesis Of N-glycan mimetics and their evaluation as C-type lectin receptor (Clr) antagonists using microarray technology | es_ES |
dc.type | info:eu-repo/semantics/doctoralThesis | es_ES |
dc.rights.holder | (c)2019 ANNA CIOCE | |
dc.identifier.studentID | 826089 | es_ES |
dc.identifier.projectID | 18040 | es_ES |
dc.departamentoes | Química orgánica I | es_ES |
dc.departamentoeu | Kimika organikoa I | es_ES |