Candida parapsilosis Colony Morphotype Forecasts Biofilm Formation of Clinical Isolates
dc.contributor.author | Gómez Molero, Emilia | |
dc.contributor.author | De la Pinta Aresti, Iker | |
dc.contributor.author | Fernández Pereira, Jordan | |
dc.contributor.author | Groß, Uwe | |
dc.contributor.author | Weig, Michael | |
dc.contributor.author | Quindós Andrés, Guillermo | |
dc.contributor.author | De Groot, Piet W. J. | |
dc.contributor.author | Bader, Oliver | |
dc.date.accessioned | 2021-01-25T11:57:39Z | |
dc.date.available | 2021-01-25T11:57:39Z | |
dc.date.issued | 2021-01-07 | |
dc.identifier.citation | Journal of Fungi 7(1) : (2021) // Article ID 33 | es_ES |
dc.identifier.issn | 2309-608X | |
dc.identifier.uri | http://hdl.handle.net/10810/49856 | |
dc.description.abstract | Candida parapsilosis is a frequent cause of fungal bloodstream infections, especially in critically ill neonates or immunocompromised patients. Due to the formation of biofilms, the use of indwelling catheters and other medical devices increases the risk of infection and complicates treatment, as cells embedded in biofilms display reduced drug susceptibility. Therefore, biofilm formation may be a significant clinical parameter, guiding downstream therapeutic choices. Here, we phenotypically characterized 120 selected isolates out of a prospective collection of 215 clinical C. parapsilosis isolates, determining biofilm formation, major emerging colony morphotype, and antifungal drug susceptibility of the isolates and their biofilms. In our isolate set, increased biofilm formation capacity was independent of body site of isolation and not predictable using standard or modified European Committee on Antimicrobial Susceptibility Testing (EUCAST) drug susceptibility testing protocols. In contrast, biofilm formation was strongly correlated with the appearance of non-smooth colony morphotypes and invasiveness into agar plates. Our data suggest that the observation of non-smooth colony morphotypes in cultures of C. parapsilosis may help as an indicator to consider the initiation of anti-biofilm-active therapy, such as the switch from azole- to echinocandin- or polyene-based strategies, especially in case of infections by potent biofilm-forming strains. | es_ES |
dc.description.sponsorship | This work was funded in part by grants or scholarships from the ZabaldUz program (Universidad del País Vasco/Euskal Herriko Unibertsitatea) to IDlP, the Consejería de Educación, Universidades e Investigación (GIC15/78 IT-990-16) of Gobierno Vasco-Eusko Jaurlaritza to GQ, the Ministerio de Economía y Competitividad (grants SAF2013-47570-P and SAF2017-86188-P, the latter co-financed by FEDER) of the Spanish government to P.G. and G.Q., and the FP7-PEOPLE-2013-ITN—Marie-Curie Action: “Initial Training Networks”: Molecular Mechanisms of Human Fungal Pathogen Host Interaction, ImResFun, MC-ITN-606786, to O.B. and U.G. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | MDPI | es_ES |
dc.relation | info:eu-repo/grantAgreement/MINECO/SAF2013-47570-P | es_ES |
dc.relation | info:eu-repo/grantAgreement/MINECO/SAF2017-86188-P, | es_ES |
dc.relation | info:eu-repo/grantAgreement/EC/FP7/606786 | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | |
dc.subject | candida parapsilosis | es_ES |
dc.subject | biofilm | es_ES |
dc.subject | colony morphology | es_ES |
dc.subject | drug susceptibility | es_ES |
dc.title | Candida parapsilosis Colony Morphotype Forecasts Biofilm Formation of Clinical Isolates | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.date.updated | 2021-01-22T15:47:04Z | |
dc.rights.holder | 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). | es_ES |
dc.relation.publisherversion | https://www.mdpi.com/2309-608X/7/1/33/htm | es_ES |
dc.identifier.doi | 10.3390/jof7010033 | |
dc.contributor.funder | European Commission | |
dc.departamentoes | Inmunología, microbiología y parasitología | |
dc.departamentoeu | Immunologia, mikrobiologia eta parasitologia |
Files in this item
This item appears in the following Collection(s)
Except where otherwise noted, this item's license is described as 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).