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dc.contributor.authorNorgaard, Louise
dc.contributor.authorOlesen, Carsten Riis
dc.contributor.authorTrojelsgaard, Kristian
dc.contributor.authorPertoldi, Cino
dc.contributor.authorNielsen, Jeppe Lund
dc.contributor.authorTaberlet, Pierre
dc.contributor.authorRuiz González, Aritz ORCID
dc.contributor.authorDe Barba, Marta
dc.contributor.authorIacolina, Laura
dc.identifier.citationScientific Reports 11(1) : (2021) // Article ID 6820es_ES
dc.description.abstractWith an accelerating negative impact of anthropogenic actions on natural ecosystems, non-invasive biodiversity assessments are becoming increasingly crucial. As a consequence, the interest in the application of environmental DNA (eDNA) survey techniques has increased. The use of eDNA extracted from faeces from generalist predators, have recently been described as "biodiversity capsules" and suggested as a complementary tool for improving current biodiversity assessments. In this study, using faecal samples from two generalist omnivore species, the Eurasian badger and the red fox, we evaluated the applicability of eDNA metabarcoding in determining dietary composition, compared to macroscopic diet identification techniques. Subsequently, we used the dietary information obtained to assess its contribution to biodiversity assessments. Compared to classic macroscopic techniques, we found that eDNA metabarcoding detected more taxa, at higher taxonomic resolution, and proved to be an important technique to verify the species identification of the predator from field collected faeces. Furthermore, we showed how dietary analyses complemented field observations in describing biodiversity by identifying consumed flora and fauna that went unnoticed during field observations. While diet analysis approaches could not substitute field observations entirely, we suggest that their integration with other methods might overcome intrinsic limitations of single techniques in future biodiversity surveyses_ES
dc.description.sponsorshipThis project was funded by Aage V. Jensen Naturfond, 15. Juni Fonden, and Aalborg Zoo Conservation Foundation (AZCF, Grant Number 3-2017). Received funding from the Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie Action (Grant Agreement No. 656697)es_ES
dc.subjectanthropogenic actionses_ES
dc.subjectnatural ecosystemses_ES
dc.subjectenvironmental DNAes_ES
dc.subjectfaeces from generalist predatorses_ES
dc.subjectbiodiversity capsuleses_ES
dc.subjectEurasian badgeres_ES
dc.subjectred foxes_ES
dc.subjectdietary compositiones_ES
dc.titleeDNA Metabarcoding for Biodiversity Assessment, Generalist Predators As Sampling Assistantses_ES
dc.rights.holderThis article is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0)es_ES
dc.rights.holderAtribución 3.0 España*
dc.contributor.funderEuropean Commission
dc.departamentoesZoología y biología celular animales_ES
dc.departamentoeuZoologia eta animalia zelulen biologiaes_ES

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