dc.contributor.author | Bozal Basterra, Laura | |
dc.contributor.author | González Santamarta, María | |
dc.contributor.author | Muratore, Verónica | |
dc.contributor.author | Martín Martín, Natalia | |
dc.contributor.author | Ercilla Eguiarte, Amaia | |
dc.contributor.author | Rodríguez Pérez, José Antonio | |
dc.contributor.author | Carracedo Pérez, Arkaitz | |
dc.contributor.author | Sutherland, James D. | |
dc.contributor.author | Barrio Olano, María Rosa | |
dc.date.accessioned | 2021-05-18T07:51:34Z | |
dc.date.available | 2021-05-18T07:51:34Z | |
dc.date.issued | 2021-04-01 | |
dc.identifier.citation | Frontiers In Cell And Developmental Biology 9 : (2021) // Article ID 624089 | es_ES |
dc.identifier.issn | 2296-634X | |
dc.identifier.uri | http://hdl.handle.net/10810/51459 | |
dc.description.abstract | LUZP1 is a centrosomal and actin cytoskeleton-localizing protein that regulates both ciliogenesis and actin filament bundling. As the cytoskeleton and cilia are implicated in metastasis and tumor suppression, we examined roles for LUZP1 in the context of cancer. Here we show that LUZP1 exhibits frequent genomic aberrations in cancer, with a predominance of gene deletions. Furthermore, we demonstrate that CRISPR/Cas9-mediated loss of Luzp1 in mouse fibroblasts promotes cell migration and invasion features, reduces cell viability, and increases cell apoptosis, centriole numbers, and nuclear size while altering the actin cytoskeleton. Loss of Luzp1 also induced changes to ACTR3 (Actin Related Protein 3, also known as ARP3) and phospho-cofilin ratios, suggesting regulatory roles in actin polymerization, beyond its role in filament bundling. Our results point to an unprecedented role for LUZP1 in the regulation of cancer features through the control of actin cytoskeleton. | es_ES |
dc.description.sponsorship | We are grateful to the Fundacion Inocente, Inocente for their support. We also acknowledge funding by the grants BFU2017-84653-P (MINECO/FEDER, EU), SEV-2016-0644 (Severo Ochoa Excellence Program), 765445-EU (UbiCODE Program), SAF2017-90900-REDT (UBIRed Program), and IT1165-19 (Basque Country Government). Additional support was provided by the Department of Industry, Tourism, and Trade of the Basque Country Government (Elkartek Research Programs) and by the Innovation Technology Department of the Bizkaia County. LB-B acknowledges POSTD19048BOZA (Fundacion Cientifica AECC). VM acknowledges PRE2018086230 (MINECO/FEDER, EU). AC acknowledges the Basque Department of education (IKERTALDE IT1106-16), the MCIU [PID2019-108787RB-I00 (FEDER/EU)], the AECC (IDEAS175CARR; GCTRA18006CARR), La Caixa Foundation (ID 100010434), under the agreement LCF/PR/HR17/and the European Research Council (Starting Grant 336343, PoC 754627, Consolidator grant 819242). CIBERONC was co-funded with FEDER funds. We are also grateful to Maria Vivanco's lab for providing reagents. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Frontiers Media | es_ES |
dc.relation | info:eu-repo/grantAgreement/MICINN/BFU2017-84653-P | es_ES |
dc.relation | info:eu-repo/grantAgreement/MICINN/PRE2018-086230 | es_ES |
dc.relation | info:eu-repo/grantAgreement/MICINN/SEV-2016-0644 | es_ES |
dc.relation | info:eu-repo/grantAgreement/EC/H2020/765445 | es_ES |
dc.relation | info:eu-repo/grantAgreement/MICINN/PID2019-108787RB-I00 | es_ES |
dc.relation | info:eu-repo/grantAgreement/EC/FP7/336343 | es_ES |
dc.relation | info:eu-repo/grantAgreement/EC/H2020/754627 | es_ES |
dc.relation | info:eu-repo/grantAgreement/EC/H2020/819242 | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.subject | LUZP1 | es_ES |
dc.subject | actin cytoskeleton | es_ES |
dc.subject | proliferation | es_ES |
dc.subject | migration | es_ES |
dc.subject | invasion | es_ES |
dc.subject | cancer | es_ES |
dc.subject | centrosome | es_ES |
dc.title | LUZP1 Controls Cell Division, Migration and Invasion Through Regulation of the Actin Cytoskeleton | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.holder | This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) | es_ES |
dc.rights.holder | Atribución 3.0 España | * |
dc.relation.publisherversion | https://www.frontiersin.org/articles/10.3389/fcell.2021.624089/full | es_ES |
dc.identifier.doi | 10.3389/fcell.2021.624089 | |
dc.contributor.funder | European Commission | |
dc.departamentoes | Bioquímica y biología molecular | es_ES |
dc.departamentoes | Genética, antropología física y fisiología animal | es_ES |
dc.departamentoeu | Biokimika eta biologia molekularra | es_ES |
dc.departamentoeu | Genetika,antropologia fisikoa eta animalien fisiologia | es_ES |