Design and Validation of a Process Based on Cationic Niosomes for Gene Delivery into Novel Urine-Derived Mesenchymal Stem Cells
dc.contributor.author | Vado Ranedo, Yerai | |
dc.contributor.author | Puras Ochoa, Gustavo | |
dc.contributor.author | Rosique, Melania | |
dc.contributor.author | Martín Plágaro, César Augusto | |
dc.contributor.author | Pedraz Muñoz, José Luis | |
dc.contributor.author | Jebari Benslaiman, Shifa | |
dc.contributor.author | Martínez de Pancorbo Gómez, María de los Angeles | |
dc.contributor.author | Zarate Sesma, Jon | |
dc.contributor.author | Pérez de Nanclares Leal, Guiomar | |
dc.date.accessioned | 2021-05-27T12:24:45Z | |
dc.date.available | 2021-05-27T12:24:45Z | |
dc.date.issued | 2021-05-11 | |
dc.identifier.citation | Pharmaceutics 13(5) : (2021) // Article ID 696 | es_ES |
dc.identifier.issn | 1999-4923 | |
dc.identifier.uri | http://hdl.handle.net/10810/51650 | |
dc.description.abstract | Background: Mesenchymal stem cells (MSCs) are stem cells present in adult tissues. They can be cultured, have great growth capacity, and can differentiate into several cell types. The isolation of urine-derived mesenchymal stem cells (hUSCs) was recently described. hUSCs present additional benefits in the fact that they can be easily obtained noninvasively. Regarding gene delivery, nonviral vectors based on cationic niosomes have been used and are more stable and have lower immunogenicity than viral vectors. However, their transfection efficiency is low and in need of improvement. Methods: We isolated hUSCs from urine, and the cell culture was tested and characterized. Different cationic niosomes were elaborated using reverse-phase evaporation, and they were physicochemically characterized. Then, they were screened into hUSCs for transfection efficiency, and their internalization was evaluated. Results: GPxT-CQ at a lipid/DNA ratio of 5:1 (w/w) had the best transfection efficiency. Intracellular localization studies confirmed that nioplexes entered mainly via caveolae-mediated endocytosis. Conclusions: In conclusion, we established a protocol for hUSC isolation and their transfection with cationic niosomes, which could have relevant clinical applications such as in gene therapy. This methodology could also be used for creating cellular models for studying and validating pathogenic genetic variants, and even for performing functional studies. Our study increases knowledge about the internalization of tested cationic niosomes in these previously unexplored cells | es_ES |
dc.description.sponsorship | This research was funded by a grant from the EITB Maratoia to G.P.d.N., C.M., and J.L.P. (BIO16/ER/005), from the University of the Basque Country UPV/EHU to Y.V. (PIF17/29), and from the Basque Government to S.J.-B. (PRE_2017-1-0365). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | MDPI | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | |
dc.subject | urine-derived mesenchymal stem cells | es_ES |
dc.subject | primary cell culture | es_ES |
dc.subject | nonviral vectors | es_ES |
dc.subject | cationic niosomes | es_ES |
dc.subject | gene therapy | es_ES |
dc.title | Design and Validation of a Process Based on Cationic Niosomes for Gene Delivery into Novel Urine-Derived Mesenchymal Stem Cells | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.date.updated | 2021-05-24T15:06:36Z | |
dc.rights.holder | 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). | es_ES |
dc.relation.publisherversion | https://www.mdpi.com/1999-4923/13/5/696/htm | es_ES |
dc.identifier.doi | 10.3390/pharmaceutics13050696 | |
dc.departamentoes | Bioquímica y biología molecular | |
dc.departamentoeu | Biokimika eta biologia molekularra |
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Except where otherwise noted, this item's license is described as 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).