dc.description.sponsorship | The following reagents were obtained through the
NIH AIDS Reagent Program, Division of AIDS, National Institute of Allergy and
Infectious Diseases: TZM-bl cells (ARP-8129; contributed by Dr. John C. Kappes
and Dr. Xiaoyun Wu); anti–HIV-1 gp160 monoclonal antibody (N6/
PGDM1400x10E8v4) (ARP-13390; contributed by Drs. Ling Xu and Gary Nabel);
HIV-1 NL4-3 ΔEnv Vpr luciferase reporter vector (pNL4-3.Luc.R-E-) (ARP-3418;
contributed by Dr. Nathaniel Landau and Aaron Diamond); plasmids pcDNA3.1
D/V5-His TOPO-expressing HIV-1 Env/Rev (ARP-11017, ARP-11018, ARP-11024,
and ARP-11022; contributed by Drs. David Montefiori, Feng Gao, and Ming Li);
plasmid pcDNA3.1(+)-expressing HIV-1 Env/Rev (ARP-11037; contributed by
Drs. B. H. Hahn and D. L. Kothe); plasmid pcDNA3.1 D/V5-His TOPO-expressing
HIV-1 Env/Rev (ARP-11308; contributed by Drs. D. Montefiori, F. Gao, C. Wil-
liamson, and S. Abdool Karim); plasmid pcDNA3.1 V5-His TOPO-expressing
HIV-1 Env/Rev (ARP-11309; contributed by Drs. B. H. Hahn, Y. Li, and J. F. Sala-
zar-Gonzalez); HIV-1 BG505 Env expression vector (BG505.W6M.ENV.C2) (ARP-
11518; contributed by Dr. Julie Overbaugh); HIV-1 Env expression vector
(CRF02_AG clone 257) (ARP-11599; contributed by Drs. D. Ellenberger, B. Li, M.
Callahan, and S. Butera); plasmid pcDNA3.1 V5-His TOPO-expressing HIV-1
CNE8 Env (ARP-12653; contributed by Drs. Linqi Zhang, Hong Shang, David
Montefiori, Tsinghua University (Beijing, China), China Medical University (Bei-
jing, China), and Duke University (Durham, NC); HIV-1 SF162 gp160 expression
vector (ARP-10463; contributed by Drs. Leonidas Stamatatos and Cecilia Cheng-
Mayer); plasmid pcDNA3.1 V5-His TOPO-expressing HIV-1 Env/Rev (ARP-11034;
contributed by Drs. B. H. Hahn, X. Wei, and G. M. Shaw); plasmid pcDNA3.1/V5-
His TOPO-expressing HIV Env/Rev (ARP-11038; contributed by Drs. B. H. Hahn
and D. L. Kothe); plasmid pcDNA3.1 V5-His TOPO-expressing HIV-1 Env/Rev
(ARP-11310; contributed by Drs. B. H. Hahn, Y. Li, and J. F. Salazar-Gonzalez);
HIV-1 Env expression vector (p16845 env) (ARP-11503; contributed by Drs. R.
Paranjape, S. Kulkarni, and D. Montefiori); HIV-1 1054 Env expression vector
(p1054.TC4.1499) (ARP-11561) and 6244 Env expression vector
(p6244_13.B5.4576) (ARP-11566; contributed by Drs. Beatrice H. Hahn, Brandon
F. Keele, and George M. Shaw); HIV-1 ZM246F Env expression vector
(pZM246F_C1G) (ARP-11830; contributed by Dr. Beatrice Hahn); HIV-1 Env
expression vector (CRF02_AG clone 278) (ARP-11605; contributed by Drs.
Michael Thomson, Ana Revilla, Elena Delgado, David Montefiori, Sonia P erez
Castro, Centro Nacional de Microbiologia, Instituto de Salud Carlos III (Majada-
honda, Madrid, Spain), Complejo Hospitalario Santa Mar ıa Madre (Orense,
Spain), Duke University (Durham, NC), and the CAVD; and NL4-3 Env expression
vector (pDOLHIVenv) (from Dr. Eric Freed and Dr. Rex Risser). The following
reagents were kindly provided by CAVD: X2988, ZM106.9, and 3817. We thank
S. Tabruyn and F. Arbogast for their assistance with in vivo studies. We thank
the SickKids-University Health Network Flow Cytometry Facility. This work wassupported by Natural Sciences and Engineering Research Council of Canada
(NSERC) Discovery Grant 6280100058 (J.-P.J.) and by Operating Grant PJ4-
169662 from the Canadian Institutes of Health Research (CIHR; B.T. and J.-P.J.).
This research was also supported by the European Union’s Horizon 2020
research and innovation program under Marie Sklodowska-Curie Grant
790012 (E.R.), a Hospital for Sick Children Restracomp Postdoctoral Fellowship
(C.B.A.), an NSERC postgraduate doctoral scholarship (T.Z.), a predoctoral fel-
lowship from the Basque Government (PRE_2019_2_0046) (S.I.), the Canadian
Institute for Advanced Research (CIFAR) Azrieli Global Scholar program (J.-P.J.),
the Ontario Early Researcher Awards program (J.-P.J.), and the CanadaResearch Chairs program (B.T. and J.-P.J.). This work was supported, in part, by
NSERC Discovery Grant RGPIN-2019-06442 and CIHR Project Grant–Priority
Announcement PJH-175379 to C.G., and a CIHR Canada Graduate Scholarship
(CGS-M) to J.B. Further support was obtained from the Spanish Ministry of Sci-
ence, Innovation and Universities (MCIU) with the support of the Spanish
Research Agency/The European Regional Development Fund (AEI/FEDER)
(RTI2018-095624-B-C21) (J.L.N.) and the Basque Government (IT1196-19)
(J.L.N.). Biophysical data were collected at the Structural & Biophysical Core
facility supported by the Canada Foundation for Innovation and Ontario
Research Fund | es_ES |