Mesenchymal stromal cells-based sustainable strategies for wound management: form valorized cell delivery systems to cell-free therapies

Abstract

In this doctoral thesis, we aimed to develop a complete biomaterial, following a circular economy thinking, which can tackle multiple stages of the wound healing process. In this sense, we designed a sponge-like scaffold composed of soy protein and ß-chitin with hemostatic properties, which can be combined with mesenchymal stromal cells (MSCs), for treating different wounds. Thanks to their anti-inflammatory, immunomodulatory and proregenerative properties, the MSCs have demonstrated to be very effective for treating multiple pathologies. However, despite the use of MSCs combined with biomaterials holds a promising potential, in this work we aimed to explore also the extracelular vesicle fraction of the MSCs secretome. Thus, this cell-free alternative presents the advantages of being; more easily handled, characterized, standardized, stored and potentially safer than MSCs, while exhibiting also therapeutic properties.