Characterization and assessment of new fibrillar collagen inks and bioinks for 3D printing and bioprinting
dc.contributor.author | García Villén, Fátima | |
dc.contributor.author | Guembe, Amaia | |
dc.contributor.author | Rey, José M. | |
dc.contributor.author | Zúñiga, Teresa | |
dc.contributor.author | Ruiz Alonso, Sandra | |
dc.contributor.author | Sáenz del Burgo Martínez, Laura | |
dc.contributor.author | Izco, Jesús M. | |
dc.contributor.author | Recalde, José I. | |
dc.contributor.author | Pedraz Muñoz, José Luis | |
dc.date.accessioned | 2023-10-05T18:14:23Z | |
dc.date.available | 2023-10-05T18:14:23Z | |
dc.date.issued | 2023-03-16 | |
dc.identifier.citation | International Journal of Bioprinting, Electronic 9(3) : 311-329 (2023) | es_ES |
dc.identifier.issn | 2424-8002 | |
dc.identifier.uri | http://hdl.handle.net/10810/62745 | |
dc.description.abstract | Collagen is a cornerstone protein for tissue engineering and 3D bioprinting due to its outstanding biocompatibility, low immunogenicity, and natural abundance in human tissues. Nonetheless, it still poses some important challenges, such as complicated and limited extraction processes, usually accompanied by batchto-batch reproducibility and influence of factors, such as temperature, pH, and ionic strength. In this work, we evaluated the suitability and performance of new, fibrillar type I collagen as standardized and reproducible collagen source for 3D printing and bioprinting. The acidic, native fibrous collagen formulation (5% w/w) performed remarkably during 3D printing, which was possible to print constructs of up to 27 layers without collapsing. On the other hand, the fibrous collagen mass has been modified to provide a fast, reliable, and easily neutralizable process. The neutralization with TRIS-HCl enabled the inclusion of cells without hindering printability. The cell-laden constructs were printed under mild conditions (50–80 kPa, pneumatic 3D printing), providing remarkable cellular viability (>90%) as well as a stable platform for cell growth and proliferation in vitro. Therefore, the native, type I collagen masses characterized in this work offer a reproducible and reliable source of collagen for 3D printing and bioprinting purposes. | es_ES |
dc.description.sponsorship | This research was funded by Viscofan (S.A.) Centro para el Desarrollo Tecnológico Industrial (CDTI) IDI-20210050 and the Basque Country Government/Eusko Jaurlaritza (Department of Education, University and Research, Consolidated Groups IT907-16). Author Sandra Ruiz-Alonso thanks the Basque Country Government for the granted fellowship PRE_2021_2_0153. José M. Rey thanks the funding from the European’s Union Horizon 2020 research and Innovation framework program (Triankle Project; Grant Agreement #952981). BioRender.com has been used as support for some figures assembly. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | AccScience Publishing | es_ES |
dc.relation | info:eu-repo/grantAgreement/EC/H2020/952981 | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.subject | type I collagen | es_ES |
dc.subject | 3D printing | es_ES |
dc.subject | 3D bioprinting | es_ES |
dc.subject | tissue engineering | es_ES |
dc.subject | bioink | es_ES |
dc.title | Characterization and assessment of new fibrillar collagen inks and bioinks for 3D printing and bioprinting | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.holder | © 2023 by the Author(s). Licensee AccScience Publishing, Singapore. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution 4.0 International License ( https://creativecommons.org/licenses/by/4.0/) | es_ES |
dc.rights.holder | Atribución 3.0 España | * |
dc.relation.publisherversion | https://accscience.com/journal/IJB/9/3/10.18063/ijb.712 | es_ES |
dc.identifier.doi | 10.18063/ijb.712 | |
dc.contributor.funder | European Commission | |
dc.departamentoes | Farmacia y ciencias de los alimentos | es_ES |
dc.departamentoeu | Farmazia eta elikagaien zientziak | es_ES |
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Except where otherwise noted, this item's license is described as © 2023 by the Author(s). Licensee AccScience Publishing, Singapore. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution 4.0 International License ( https://creativecommons.org/licenses/by/4.0/)