Unravelling molecular recognition events between glycans and proteins using advanced NMR Techniques

Abstract

Glycans are ubiquitous molecules in nature. They participate in a plethora of functions, they cover the surface of cells as integral constituents of complex glycoconjugates (glycoproteins, and glycolipids) forming the glycocalyx. The molecular recognition of glycans by lectins is a finely tuned process where even the most minimal change within the recognized glycan structure can lead to drastic changes within the meticulously orchestrated symphony of life. For that reason, understanding the structure, dynamics, and interactions of glycans is of paramount importance for being able to modulate biological events related to health and disease. In this Thesis, I have employed a synergic combination of advanced NMR spectroscopy and X-ray crystallography methods with biophysical techniques, assisted by computational chemistry tools to elucidate the intricate process of molecular recognition of glycans.